Overview:

GST or glutathione S-transferase family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are used to create the so-called GST gene fusion system (1). These proteins are capable of multiple reactions with a multitude of substrates.GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small.GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione (1).

Gene Aliases:

Glutathione S-transferase

Genbank Number:

U13850

References:

1. Beckett, G.J. and Hayes, J.D. Glutathione S-transferase measurements and liver disease in man. J. Clin. Biochem. Nutr. 1987, 2:1-24.